Dr. Chandra Srinivasan
Associate Professor of Biochemistry
Office    DBH-141  
Phone    (714) 278-5439  
Lab    DBH-141  
Phone    (714) 278-3682  
E-mail    chandra@fullerton.edu  
Web Site     faculty.fullerton.edu/chandra/  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

 

 

 
Teaching

CHEM 303 (A - C), GE Biotechnology                      

CHEM/BIO 477, Advances in Biotechnology       

CHEM 422, General Biochemistry Laboratory               

CHEM 423A, General Biochemistry Lecture                 

CHEM 541, Protein Biochemistry 

CHEM 542, Nucleic Acid Biochemistry 

 
Education

Postdoctoral    1998-2002    UCLA, Los Angeles, CA
Postdoctoral    1996-1998    University of Utah, Salt Lake City, UT
Ph.D.              1996              Loyola University Chicago, Chicago, IL
M.Sc.             1991              St. Joseph's College, Bangalore, India
B.Sc.              1989              St. Joseph's College, Bangalore, India
 

Research

Oxidative Stress, Metals, and Aging

My laboratory has two main goals. One is to understand the importance of the antioxidant enzyme superoxide dismutase (SOD) in oxidative stress and aging in the model organism Caenorhabditis elegans.  The second is to study transition metal metabolism (specifically copper, iron and manganese) in C. elegans and to assess its relevance to oxidative stress and aging.  Altered metal metabolism and oxidative stress has been linked with several human neurodegenerative disorders.  Oxidative stress is also thought to play a role in aging.  My laboratory is using various analytical, biochemical and biophysical methods to explore the contribution of SOD and metals to oxidative stress and aging.  For more details see http://faculty.fullerton.edu/chandra/

 
Recent Publications

1.         Munroe, W., Kingsley, C., Durazo, A., Gralla, E. B., Imlay, J. A., Srinivasan, C., and Valentine, J. S. (2007) Only one of a wide assortment of manganese-containing SOD mimicking compounds rescues the slow aerobic growth phenotypes of both Escherichia coli and Saccharomyces cerevisiae strains lacking superoxide dismutase enzymes. J Inorg Biochem., 101(11-12):1875-82. 

2.         Pate, K., Rangel, N., Fraser, B., Clement, M. H. S., and Srinivasan, C. (2006)  Measuring “Free" Iron Levels in Caenorhabditis elegans Using Low-temperature Fe(III) Electron Paramagnetic Resonance Spectroscopy.  Anal. Biochem., 358(2):199-207.

3.         Lin, Y., Hoang, H., Hsieh, S. I., Rangel, N., Foster, A. L., Sampayo, J. N., Lithgow, G. J., and Srinivasan, C. (2006)  Manganous Ion Supplementation Accelerates Wild-Type Development, Enhances Stress Resistance and Rescues the Life-Span of a Short–Lived Caenorhabditis elegans Mutant.  Free Radic. Biol. Med., 40: 1185-93.

4.         Sanchez, R. J., Srinivasan, C., Wallace, M. A., Martins, J., Munroe, W. H., Kao, T. Y., Le, K., Valentine, J. S.  (2005)  Exogenous Manganous Ion at Millimolar Levels Rescues All Known Defects of Yeast Lacking Copper-Zinc Superoxide Dismutase.  J. Bioinorg. Chem 10: 913-23.

5.         Srinivasan, C., Toon, J., Amari, L., Abukhdeir, A. M., Hamm, H., Geraldes, C. F., Ho, Y. K. & De Freitas, D. M. (2004)  Competition between lithium and magnesium ions for the G-protein transducin in the guanosine-diphosphate bound conformation.  J Inorg Biochem 98, 691-701.

6.         Jensen, L. T., Sanchez, R. J., Srinivasan, C., Valentine, J. S. & Culotta, V. C. (2004)  Mutations in Saccharomyces cerevisiae iron sulfur cluster assembly genes and oxidative stress relevant to Cu/Zn superoxide dismutase.  J Biol Chem. 279(29): 29938-43.

7.         Srinivasan, C. & Gralla, E. B. (2002)  Measurement of "free" or electron paramagnetic resonance-detectable iron in whole yeast cells as indicator of superoxide stress.  Methods Enzymol 349, 173-80.

8.         Wei, J. P., Srinivasan, C., Han, H., Valentine, J. S. & Gralla, E. B. (2001)  Evidence for a novel role of copper-zinc superoxide dismutase in zinc metabolism.  J Biol Chem 276, 44798-803.

9.         Minadeo, N., Layden, B., Amari, L. V., Thomas, V., Radloff, K., Srinivasan, C., Hamm, H. E. & de Freitas, D. M. (2001)  Effect of Li+ upon the Mg2+-dependent activation of recombinant Gialpha1.  Arch Biochem Biophys 388, 7-12.

10.       Srinivasan, C., Liba, A., Imlay, J. A., Valentine, J. S. & Gralla, E. B. (2000)  Yeast lacking superoxide dismutase(s) show elevated levels of "free iron" as measured by whole cell electron paramagnetic  resonance.  J Biol Chem 275, 29187-92.       
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